Journal: Cancer Cell

Article Title: The ERBB-STAT3 Axis Drives Tasmanian Devil Facial Tumor Disease

doi: 10.1016/j.ccell.2018.11.018

Figure Lengend Snippet:

Article Snippet: Kinase Inhibitor Drug Library , TargetMol and Cayman Chemical , Cat# L1600 and Cat# 10505 respectively.

Techniques: Recombinant, Drug discovery, DNA Methylation Assay, Gene Expression, RNA Sequencing

Journal: Journal of Cancer

Article Title: YTHDF2 inhibit the tumorigenicity of endometrial cancer via downregulating the expression of IRS1 methylated with m 6 A

doi: 10.7150/jca.54527

Figure Lengend Snippet: YTHDF2 regulated the activity of endometrial cell via targeting IRS1. ( A , B ) qRT-PCR and immunoblot analysis of IRS1 in HEC-1-A transfected with negative control (NC), IRS1 siRNA #1 or #2 as indicated for 48 h. ( C , E-H ) qRT-PCR analysis of MMP9 mRNA in HEC-1-A transfected with siRNA ( C,F ), plasmids ( E,G ) or treated with AKT inhibitor ( H ) as indicated, and stimulated with IGF for 6 h. ( D ) Immunoblot analysis of IRS1 in HEC-1-A transfected with the IRS1 plasmids for 48 h. ns: not significant; *p<0.05 (Student's t-test). Data are representative of three independent experiments (mean and s.d. of technical triplicates ( A,C,E-H ).

Article Snippet: AKT kinase inhibitor (HY-10249A) was from MCE (USA).

Techniques: Activity Assay, Quantitative RT-PCR, Western Blot, Transfection, Negative Control